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KMID : 1145120170150010045
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 1 p.45 ~ p.53
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Anti-aging Effects of P7C3 in UVA-irradiated Human Dermal Fibroblasts
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Shim Joong-Hyun
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Abstract
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Purpose: Ultraviolet (UV) radiation, specifically ultraviolet A (UVA), is known to induce photo-aging of human skin by promoting the aging phenomenon, such as skin elasticity reduction, wrinkle formation, and retardation of wound healing. Effects of P7C3, a small molecule neuroprotective drug candidate, were studied on the collagen, type I, alpha 1 (COL1A1) and matrix metallopeptidase 1 (MMP1) production in normal human face derived dermal fibroblasts (NHDFs), as a raw material of cosmetics.
Methods: Cytotoxicity of the UVA on NHDFs was determined by cell counting kit-8 (CCK-8) assay. COL1A1 and MMP1 mRNA expressions were confirmed by two types of polymerase chain reaction (PCR), reverse transcription PCR and real-time PCR. In addition, wound healing assay was performed to demonstrate migration activity in P7C3-treated cells.
Results: Increased COL1A1 production was observed by real-time PCR and COL1A1-enzyme linked immunosorbent assay (ELISA). The MMP1 expression was reduced by using UVA (5 J/cm2)-treated NHDFs. Furthermore, a scratch wound healing assay showed that P7C3 enhanced the migratory properties of UVA-irradiated NHDFs.
Conclusion: The effects of P7C3 in improving aging skin were demonstrated in the cell efficacy test, and P7C3 is expected to have a value as an effective anti-aging material for functional cosmetics.
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KEYWORD
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P7C3, Ultraviolet, Collagen, Matrix metallopeptidase 1, Aging
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